Branson Accessories for 250 and 450

Continuous Flow Attachment

Continuos Flow Attachment

Applications:

• Production of vaccines
• Emulsification of immiscible liquids (with or w/o surfactants)
• Removal of cell walls
• Dispersing metal oxides in solvents

The stainless steel, in-line continuous flow cell uniformly processes low-viscosity solutions at rates up to 10 GPH (38LPH). It can be used to emulsify, disperse, and homogenize by pumping a solution through a zone of intense ultrasonic activity. The degree of processing is controlled by varying the amplitude of the ultrasonic horn and the flow rate. Some solutions may require recirculation until the desired results are obtained.

The continuous flow attachment incorporates a cooling jacket through which a suitable cooling liquid can be circulated to retard heat build-up during extended operation. The attachment may also be sealed in a closed system to assure sterile conditions and inhibit contamination when working with infectious materials.

Overall dimensions: 5”L x 3.8” D (includes port)

Continuous Flow Attachment*

Tapered Microtip

Microtips

Applications

• Tapered microtips—processing spores, fungi, yeast , muscle, and connective tissue
• Stepper microtip—recommended for red and white blood cells, tissue culture cells, HELA cells, and cells which have a low to medium resistance to breakage

Microtip probes are available in tapered and stepped configurations and are designed to process samples contained in small diameter vessels at extremely high intensity (refer to charts for recommended volumes).

Tapered microtips screw into standard 1/2" step horns with tapped ends. The double stepped microtip consists of a coupler and a stepped tip and screws directly into the converter. It has lower amplitude than the tapered tip, but is capable of reaching into small diameter vessels of greater depth and can process solutions down to 0.5 ml.

WARNING: because of their high amplitude, microtips are highly stressed and can break if operated in air or at an output control setting greater than 7.

Uncooled Sealed-atmosphere Treatment Chamber

Cooled Sealed-atmosphere Treatment Chamber

Sealed-Atmosphere Treatment Chamber

Applications:

• Pressure sonification to increase cavitation intensity
• Sonify in inert environments
• Hazardous material sonification
• Capture released gasses

This accessory is designed for batch treatment of infectious materials in an isolated environment with an inert atmosphere and ice-bath cooling, if desired. The solid stainless steel chamber screws onto the horn with a neoprene O-ring seal and can be autoclaved. It is equipped with hose connections above the liquid level to permit purging with an inert gas and for filling/emptying without breaking the atmospheric seal. Standard chambers will permit treatment of 3-10 ml, 6-15 ml, or 25-50 ml volumes and can be obtained with or without a built-in cooling chamber.

Acoustic Sound Enclosure for the Sonifier

Sonifier Sound Enclosure

• Transparent door offers viewing convenience
• Decibel rating can be lowered by 20-25dB
• Document drawer and tool shelf
• Easy to clean
• Easy access to the coolant tubing

Although ultrasonics is above the audible range of the human ear, mechanical noise often occurs when liquids are treated ultrasonically. This noise may be disturbing if the exposure lasts for prolonged periods of time. 

Operating the Sonifier in the Enclosure can minimize mechanical noise produced by ultrasonic processing, allowing the ability to lower the dB rating by 20-25dB. The sturdy cabinet is lined with waterproof, sound-abating material, which is impervious to most solutions or laboratory regents. The enclosure has openings at the top and bottom which allows easy access of either a microtip or cup horn.

The sample position can be adjusted by placing it on a plate which can be clamped on a column at any height. A transparent door enables viewing of the process while limiting noise to an acceptable level. An external shelf is located on the right side of the unit which accommodates wrenches, plus four ½ inch tips and three ¾ Sonotrodes, and a document storage drawer is located on the left.

Rosette Cooling Cell

Rosette Cooling Cell

Branson’s Rosette Cooling Cell provides a unique flow pattern of substances for exposure to ultrasonic energy during circulation through the cell. When it is immersed in a cooling bath, the enlarged glass surface area and circulation through the side arms provide an efficient means of heat exchange. Available in three sizes.

Horns

Standard Disruptor Horn

Disruptor Horns

Horns (probes) transmit ultrasonic energy into a solution. The dimensions of the horn and the output control setting determine the amount of amplitude (tip movement) and degree of ultrasonic activity in the liquid. Generally, the smaller the tip diameter, the higher the amplitude. Larger tip diameters have less amplitude but can accommodate larger volumes.

A step horn with a threaded body enables the attachment of various screw-on accessories, such as a continuous flow cell. Tapped horn ends enable attachment of microtips. Tapped horns are supplied with removable flat tips.

High gain horns cannot be used with screw-on accessories but provide higher amplitude at 3/4" diameter than the same diameter step horn.

Exponential horns have lower tip amplitude but can withstand higher tip loading. Best suited for applications requiring the compressing of solids such as powders and tissue.

All Sonifier horns and tips are fabricated from titanium. Please note the charted amplitude ratings and liquid volumes when selecting horns.

Flow-thru Horn

Flow-Thru Horns

Designed for pharmaceutical research, the flow-thru horn has two inputs or orifices at the non-vibrating nodal point of the horn. A premixed substance is fed through one of the inputs while the horn is ultrasonically activated. Because two inputs are available, two different types of material can be treated simultaneously. The processed solution exits at the tip of the horn. The horn may be used as a continuous flow device to collect the solution in one large vessel.

Applications:

• Mixing
• Emulsification
• Dispersion
• Formation of mists and fogs

Cup Horn

Cup Horn

The cup horn enables sonification of a sample without the horn coming in contact with the solution. It can simultaneously process a number of sealed vials or test tubes with identical parameters.

Applications:

• Liposomes preparation
• Process samples in complete isolation
• Precludes the possibly of any titanium migration
• Ideal for minute volume processing — down to 0.3 ml
• Processing of viruses and radioactive materials

High-intensity Cup Horn

High-intensity Cup Horn

Branson’s water-jacketed cup horn ultrasonically processes samples in a test tube or a sealed vessel, thereby isolating the horn from the solution. One-piece solid titanium construction is utilized, allowing isolation of samples for treatment. High-intensity cavitation is possible due to the horn’s concaved shape. This design permits disruption of many cells and is used for emulsification, dispersion, creation of lipid vesicles, suspension, homogenization, and degassing. Nalgon fitting for the coolant port is supplied.

Applications:

• Blood chemistry
• Lipid vesicle preparation
• Tablet dispersion
• Waste homogenization
• HPLC solvent degassing
• Pigment dispersion
• Tissue culture
• Infectious solutions