SmartGlow Nucleic Acid Stains Accuris

Benefits of SmartGlow E4500-PS

• 20,000x concentration
• Replaces ethidium bromide (EB) in agarose and polyacrylamide gel electrophoresis; can also be used with UV light visualization
• Light emission: green fluorescence when bound to dsDNA or ssDNA; red fluorescence
• Excitation peaks: 490nm (blue); 290nm (UV)

Protocol for SmartGlow E4500-PS

• Use with prepared 100ml of agarose or polyacrylamide solution
• Add 5µl of SmartGlow E4500-PS Pre-Stain to gel solution prior to pouring gels
• For increased sensitivity and improved detection of small quantities of nucleic acid, add SmartGlow PS to running buffer at ratio of 5µl per 100 ml
• When electrophoresis is complete, view gel using SmartBlue Blue Light Transilluminator

Benefits of SmartGlow E4500-LD

• 6x concentration
• Add directly to samples
• Direct addition to samples eliminates chance of contaminating containers and gels
• Excitation peaks: 490nm (blue); 290nm (UV)

Protocol for SmartGlow E4500-LD

• Prepare 100ml of agarose or polyacrylamide solution
• Pour gels
• Mix SmartGlow LD with DNA markers and samples at a 1 to 5 (dye to sample) dilution
• After electrophoresis, view gel using SmartBlue Blue Light Transilluminator