• Manufacturer SKU: MAKB
• Unit of Measure: EA
Provide a comprehensive solution for cDNA synthesis from mRNA or bacterial mRNA templates. | 3033-61 displayed
Arrayit MiniAmp Bacterial mRNA Amplification Kit, 10 Reactions
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Summary
- Includes all of the necessary components for first strand cDNA synthesis, second strand synthesis and amplification of mRNA targets
- Yield: 10 reactions
- KIT CONTAINS:
- Enzyme Mix 1: 16.5 μl in 0.5 ml tube
- TR Booster (Enhancer solution): 50 μl in 0.5 ml tube
- Reaction buffer 1: 50 μl in 0.5 ml tube
- DNTP Mix: 40 μl in 0.5 ml tube
- DTT: 50 μl in 0.5 ml tube
- Primer 1 Mix: 11 μl in 0.5 ml tube
- Primer 2: 11 μl in 0.5 ml tube
- Primer 3: 22 μl in 0.5 ml tube
- Primer 4AS: 11 μl in 0.5 ml tube
- Enzyme Mix 2: 11 μl in 0.5 ml tube
- Reaction Buffer 2: 150 μl in 0.5 ml tube
- DNA Binding Buffer: 1.1 ml in 2 ml tube
- DNA Wash Buffer Concentrate: 0.5 ml in 8 ml bottle
- DNA Micro Column (without cap) : 10 each
- Elution Tube (1.5 ml) : 10 each
- 2 ml Wash Tube: 10 each
- Nuclease Free Water: 1.5 ml in 2 ml tube
- Enzyme Mix 3: 22 μl in 0.5 ml tube
- NTP Mix: 79.2 μl in 0.5 ml tube
- Reaction Buffer 3: 22 μl in 0.5 ml tube
- DNAse: 11 μl in 0.5 ml tube
- Sodium Acetate Solution: 50 μl in 0.5 ml tube
- Linear Acrylamide: 20 μl in 0.5 ml tube
- For use with Arrayit EC40K Escherichia coli K12 and BA40K Bacillus subtilis subsp. Subtilis str. 168 Whole Genome Chips
- Complete kit for E. Coli and other bacterial and prokaryotic RNA amplification
- Includes all of the necessary components for first strand cDNA synthesis, second strand synthesis and amplification of mRNA targets
- Huge incremental yield in in-vitro transcription with optimized T7 reaction, a proprietary blend of enhancers to augment the polymerase activity
- Generates 5 to 50 μg of amplified sense strand mRNA
- Optimized to use 50 ng to 1μg of total cellular RNA as starting material
Specifications
• Manufacturer SKU: MAKB
• Unit of Measure: EA
• Weight, Product: 2 lb
• Weight, Shipping: 4 lb
• Package Type: Box